TY  - JOUR
ID  - open3319
UR  - http://crdd.osdd.net/open/3319/
IS  - 1
A1  - Khajuria, Akhil
A1  - Alajangi, Hema K
A1  - Sharma, Akanksha
A1  - Kaur, Harinder
A1  - Sharma, Prakriti
A1  - Negi, Sushmita
A1  - Kumari, Laxmi
A1  - Trivedi, Manisha
A1  - Yadav, Ashok Kumar
A1  - Kumar, Robin
A1  - Raghuvanshi, Rajeev Singh
A1  - Kaur, Indu Pal
A1  - Tyagi, Rajeev K
A1  - Jaiswal, Pradeep Kumar
A1  - Lim, Yong-Beom
A1  - Barnwal, Ravi Pratap
A1  - Singh, Gurpal
Y1  - 2024/09//
N2  - Breast cancer is one of the leading causes of death among women globally, making its diagnosis and treatment challenging. The use of nanotechnology for cancer diagnosis and treatment is an emerging area of research. To address this issue, multiwalled carbon nanotubes (MWCNTs) were ligand exchanged with butyric acid (BA) to gain hydrophilic character. The successful functionalization was confirmed by FTIR spectroscopy. Surface morphology changes were observed using SEM, while TEM confirmed the structural integrity of the MWCNTs after functionalization. Particle size, zeta potential, and UV spectroscopy were also performed to further characterize the nanoparticles. The breast cancer aptamer specific to Mucin-1 (MUC-1) was then conjugated with the functionalized MWCNTs. These MWCNTs successfully targeted breast cancer cells (MDA-MB-231) as examined by cellular uptake studies and exhibited a reduction in cancer-induced inflammation, as evidenced by gene transcription (qPCR) and protein expression (immunoblotting) levels. Immunoblot and confocal-based immunofluorescence assay (IFA) indicated the ability of CNTs to induce photothermal cell death of MDA-MB-231 cells. Upon imaging, cancer cells were effectively visualized due to the MWCNTs' ability to act as magnetic resonance imaging (MRI) contrast agents. Additionally, MWCNTs demonstrated photothermal capabilities to eliminate bound cancer cells. Collectively, our findings pave the way for developing aptamer-labeled MWCNTs as viable ``theranostic alternatives'' for breast cancer treatment.
PB  - Springer Science and Business Media LLC
JF  - Discov. Nano
VL  - 19
KW  - Aptamer; Breast cancer; MWCNT; Mucin; Nanotechnology; Theranostics
TI  - Theranostics: aptamer-assisted carbon nanotubes as MRI contrast and photothermal agent for breast cancer therapy
ER  -