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primary topic: Characterization of multiple polycyclic aromatic hydrocarbons (PAHs) degrading Achromobacter xylosoxidans IITR150: Novel insights from whole genome analysis

Characterization of multiple polycyclic aromatic hydrocarbons (PAHs) degrading Achromobacter xylosoxidans IITR150: Novel insights from whole genome analysis
type: AcademicArticle
type: Article
abstract: Polycyclic aromatic hydrocarbons (PAHs) are biodegraded primarily by bacterial activities from polluted environments. From an oily sludge disposals site an Achromobacter xylosoxidans bacterium (designated as strain IITR150) was isolated with capability to grow and degrade multiple PAHs. Strain IITR150 was found to utilize naphthalene, phenanthrene, anthracene, benz(a)pyrene, benz(a)anthracene, and fluoranthene as the carbon source for its growth. Initially, the bacterium was screened based on its ability to turn indole into indigo and formed metabolites of the indole pathway such as indoxyl, isatin and indirubin. A 1014 bp gene encoding for a naphthalene 1,2-dioxygenase was found to initiate the biodegradation of naphthalene to have 42.85% homology to reported genes. Whole genome sequencing of A. xylosoxidans IITR150 had showed 5.9 Mb genome size containing 5625 predicted CDS. Among 5625 predicted CDS, cytochrome P450, catechol 2,3-dioxygenase (catE), naphthalene 1,2-dioxygenase system ferredoxin-NAD(P) ( +) reductase component (nahA), naphthalene 1,2-dioxygenase system, and ferredoxin components (nahB) genes were identified that indicated the metabolic potential of the bacterium for utilization of different aromatic compounds. Besides identification of PAHs degrading genes along with genomic insights suggests the robustness of the bacteria. Also, a cytochrome P450 (cytP450) enzymes that play a pivotal role in the detoxification of xenobiotics was found in IITR150, when aligned with three other cytP450 showed over 99.0% similarity at amino acid level. In conclusion, experimental and whole genome analysis provide capabilities of strain IITR150 involved in PAHs metabolism which could be useful in biodegradation of mixed PAHs contaminated soils. Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-024-01407-2.
date: 2025-12
volume: 65
issue: 4
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Springer Science and Business Media LLC
type: Organization

Indian J. Microbiol.
type: Collection

Gulfishan Khan
type: Person
given name: Gulfishan
family name: Khan

Srikrishna Subramanian
type: Person
given name: Srikrishna
family name: Subramanian

Saurabh Singh
type: Person
given name: Saurabh
family name: Singh

Varsha Tripathi
type: Person
family name: Varsha Tripathi

Natesan Manickam
type: Person
given name: Natesan
family name: Manickam

HTML Summary of #3320 Characterization of multiple polycyclic aromatic hydrocarbons (PAHs) degrading Achromobacter xylosoxidans IITR150: Novel insights from whole genome analysis
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primary topic: Characterization of multiple polycyclic aromatic hydrocarbons (PAHs) degrading Achromobacter xylosoxidans IITR150: Novel insights from whole genome analysis