<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Stoichiometry of ligand binding and role of C-terminal lysines in Mycobacterium tuberculosis and human GAPDH multifunctionality"^^ . "Glyceraldehyde-3-phosphate-dehydrogenase (GAPDH; EC1.2.1.12) has several functions in Mycobacterium tuberculosis (Mtb) and the human host. Apart from its role in glycolysis, it serves both as a cell surface and a secreted receptor for plasmin(ogen) (Plg/Plm), transferrin (Tf), and lactoferrin (Lf). Plg sequestration by Mtb GAPDH facilitates bacterial adhesion and tissue invasion, while an equivalent interaction with host GAPDH regulates immune cell migration. In both, host and microbe, internalization of Tf/Lf-GAPDH complexes serves as a route for iron acquisition. To date, the structure of Mtb GAPDH or the residues involved in these moonlighting interactions have not been identified. This study provides the first known X-ray crystal structure of Mtb GAPDH. Through further mutagenesis and functional assays, we found that the C-terminal lysines of Mtb and human GAPDH affect enzyme activity and ligand binding. We also establish the stoichiometry of Plg, Tf and Lf interactions with the GAPDH tetramer. Lastly, molecular simulation studies reveal the interactions of the C-terminal lysine residues."^^ . "2024-12" . "291" . "23" . . "FEBS J."^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Subramanian"^^ . "Karthikeyan"^^ . "Subramanian Karthikeyan"^^ . . "Sriraj"^^ . "Seth"^^ . "Sriraj Seth"^^ . . "Rajender"^^ . "Kumar"^^ . "Rajender Kumar"^^ . . "Adarsh"^^ . "Kumar"^^ . "Adarsh Kumar"^^ . . "Manoj"^^ . "Raje"^^ . "Manoj Raje"^^ . . "Ajay"^^ . "Kumar"^^ . "Ajay Kumar"^^ . . "Chaaya"^^ . "Iyengar Raje"^^ . "Chaaya Iyengar Raje"^^ . . "Himanshu"^^ . "Malhotra"^^ . "Himanshu Malhotra"^^ . . "Vishant Mahendra"^^ . "Boradia"^^ . "Vishant Mahendra Boradia"^^ . . "Prabha"^^ . "Garg"^^ . "Prabha Garg"^^ . . . . . "HTML Summary of #3321 \n\nStoichiometry of ligand binding and role of C-terminal lysines in Mycobacterium tuberculosis and human GAPDH multifunctionality\n\n" . "text/html" . .