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primary topic: Visualizing the nucleating and capped states of f-actin by Ca2+-gelsolin: Saxs data based structures of binary and ternary complexes

Visualizing the nucleating and capped states of f-actin by Ca2+-gelsolin: Saxs data based structures of binary and ternary complexes
type: AcademicArticle
type: Article
abstract: Structural insight eludes on how full-length gelsolin depolymerizes and caps filamentous (F-)actin, while the same entity can nucleate polymerization of G-actins. Analyzing small angle X-ray scattering (SAXS) data, we deciphered assemblies which enable these contrasting processes. Mixing Ca2+-gelsolin with F-actin in high salt F-buffer resulted in depolymerization of ordered F-actin rods to smaller sized species which became monodispersed upon dialysis with low salt G-buffer. These entities were the ternary (GA2) and binary (GA) complexes of gelsolin and actin with radius of gyration and maximum linear dimension of 4.55 and 4.68 nm, and 15 and 16 nm, respectively. Using size exclusion chromatography in-line with SAXS, we confirmed that initially GA and GA2 species are formed as seen upon depolymerization of F-actin followed by dialysis. Interestingly, while GA2 could seed formation of native-like F-actin in both G- and F-buffer, GA failed in G-buffer. Thus, GA2 and GA are the central species formed via depolymerization or towards nucleation. SAXS profile referenced modeling revealed that: 1) in GA, actin is bound to the C-terminal half of gelsolin, and 2) in GA2, second actin binds to the open N-terminal half accompanied by dramatic rearrangements across g1-g2 and g3-g4 linkers.
date: 2024-10
volume: 278
issue: Pt 1
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Elsevier BV
type: Organization

Int. J. Biol. Macromol.
type: Collection

Vikas Choudhary
type: Person
given name: Vikas
family name: Choudhary

Amin Sagar
type: Person
given name: Amin
family name: Sagar

Ashish
type: Person
family name: Ashish

Renu Garg
type: Person
given name: Renu
family name: Garg

Yawar Mir
type: Person
given name: Yawar
family name: Mir

Nagesh Peddada
type: Person
given name: Nagesh
family name: Peddada

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primary topic: Visualizing the nucleating and capped states of f-actin by Ca2+-gelsolin: Saxs data based structures of binary and ternary complexes