@article{open3343,
          volume = {14},
          number = {1},
           month = {July},
          author = {Shardulya Shukla and Nitish Kumar and Pushpender Bhardwaj and Priyanka Pandita and Manoj Kumar Patel and Mohan Singh Thakur and Raj Kumar and Monisha Rawat and Shweta Saxena},
           title = {Effect of cold arid high-altitude environment on bioactive phytochemical compounds of organically grown Brassicaceae vegetables for nutri-health security in mountainous regions},
       publisher = {Springer Science and Business Media LLC},
            year = {2024},
         journal = {Sci. Rep.},
           pages = {15976},
        keywords = {Antioxidant; Brassicaceae; Organic farming; Phytochemical compounds; Secondary metabolites},
             url = {http://crdd.osdd.net/open/3343/},
        abstract = {High-altitude (HA) environment presents immense physiological adversities for humans that have been overcome by supplementing bio-active phytochemicals from functional foods that support and accelerate acclimatization under these extreme environmental conditions. Several agricultural interventions have been investigated to enhance the phytochemical content in vegetables however; these studies have been limited to low-altitude (LA) regions only. In view of an existing knowledge gap, current work is designed to compare the phytochemical compositions of HA and LA-grown Brassicaceae vegetables (cabbage, cauliflower, knol-khol, and radish) using organic treatments via farm yard manure (FYM) and Azotobacter. The open field study was conducted as a two-factorial randomized block design. The first factor was treatment (T1-FYM, T2-Azotobacter, T3-FYM + Azotobacter, and T4-control) while the second was locations (HA and LA). Among all these treatments, the application of treatment T3 in HA-grown cabbage showed the highest total phenolic content (TPC; 9.56 {\ensuremath{\mu}}g/mg), total flavonoids content (TFC; 14.48 {\ensuremath{\mu}}g/mg), and antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH; 85.97\%) and ferric reducing antioxidant power (FRAP; 30.77 {\ensuremath{\mu}}g/mg) compared to LA grown samples. Reverse Phase high performance liquid chromatography (RP-HPLC) analysis showed that treatment T3 at HA led to significantly high kaempferol (0.92 {\ensuremath{\mu}}g/mg) and sulforaphane (8.94 {\ensuremath{\mu}}g/mg) contents in cabbage whereas, indole-3-carbinol (1.31 {\ensuremath{\mu}}g/mg) was higher in HA grown cauliflower. The present study provides scientific evidence for the enrichment of health-promoting phytochemical compounds in Brassicaceae vegetables grown with T3 treatment specifically at HA.}
}