@article{open3349,
          volume = {16},
          number = {15-16},
           month = {July},
          author = {Anunay Sinha and Sanjeev Khosla},
           title = {Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus},
       publisher = {Informa UK Limited},
            year = {2024},
         journal = {Epigenomics},
           pages = {1081--1096},
        keywords = {epigenetics; histone modifications; host-microbe Interaction; lactobacillus; m6A methylation},
             url = {http://crdd.osdd.net/open/3349/},
        abstract = {Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials \& methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.}
}