%0 Journal Article
%A Sinha, Anunay
%A Khosla, Sanjeev
%D 2024
%F open:3349
%I Informa UK Limited
%J Epigenomics
%K epigenetics; histone modifications; host-microbe Interaction; lactobacillus; m6A methylation
%N 15-16
%P 1081-1096
%T Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus
%U http://crdd.osdd.net/open/3349/
%V 16
%X Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.