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primary topic: Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus

Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus
type: AcademicArticle
type: Article
abstract: Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.
date: 2024-07
volume: 16
issue: 15-16
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Informa UK Limited
type: Organization

Epigenomics
type: Collection

Anunay Sinha
type: Person
given name: Anunay
family name: Sinha

Sanjeev Khosla
type: Person
given name: Sanjeev
family name: Khosla

HTML Summary of #3349 Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus
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primary topic: Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus