TY  - JOUR
ID  - open3349
UR  - http://crdd.osdd.net/open/3349/
IS  - 15-16
A1  - Sinha, Anunay
A1  - Khosla, Sanjeev
Y1  - 2024/07//
N2  - Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.
PB  - Informa UK Limited
JF  - Epigenomics
VL  - 16
KW  - epigenetics; histone modifications; host-microbe Interaction; lactobacillus; m6A methylation
TI  - Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus
SP  - 1081
EP  - 1096
ER  -