creators_name: Sinha, Anunay
creators_name: Khosla, Sanjeev
type: article
datestamp: 2026-02-07 11:55:18
lastmod: 2026-02-07 11:55:18
metadata_visibility: show
title: Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus
keywords: epigenetics; histone modifications; host-microbe Interaction; lactobacillus; m6A methylation
abstract: Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.
date: 2024-07
publication: Epigenomics
volume: 16
number: 15-16
publisher: Informa UK Limited
pagerange: 1081-1096
citation:   Sinha, Anunay and Khosla, Sanjeev  (2024) Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus.  Epigenomics, 16 (15-16).  pp. 1081-1096.