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primary topic: Mechanistic insights into granule-bound starch synthase I (GBSSI.L539P) allele in high amylose starch biosynthesis in wheat (Triticum aestivum L.)

Mechanistic insights into granule-bound starch synthase I (GBSSI.L539P) allele in high amylose starch biosynthesis in wheat (Triticum aestivum L.)
type: AcademicArticle
type: Article
abstract: Amylose fraction of grain starch is correlated with a type of resistant starch with better nutritional quality. Granule-bound starch synthase I (GBSSI) is the known starch synthase, responsible for elongation of linear amylose chains. GBSSI expression, activity, and binding to starch and other proteins are the key factors that can affect amylose content. Previously, a QTL, qhams7A.1 carrying GBSSI mutant allele, was identified through QTL mapping using F2 population of the high amylose mutant line, 'TAC 75'. This high amylose mutant line has >2-fold higher amylose content than wild variety 'C 306'. In this study, we characterized this novel mutant allele, GBSSI.L539P. In vitro starch synthase activity of GBSSI.L539P showed improved activity than the wild type (GBSSI-wt). When expressed in yeast glycogen synthase mutants (Δgsy1gsy2), GBSSI-wt and GBSSI.L539P partially complemented the glycogen synthase (gsy1gsy2) activity in yeast. Structural analysis by circular dichroism (CD) and homology modelling showed no significant structural distortion in the mutant enzyme. Molecular docking studies suggested that the residue Leu539 is distant from the catalytic active site (ADP binding pocket) and had no detectable conformational changes in active site. Both wild and mutant enzymes were assayed for starch binding in vitro, and demonstrating higher affinity of the GBSSI.L539P mutant for starch than the wild type. The present study indicated that distant residue (L539P) influenced GBSSI activity by affecting its starch-binding ability. Therefore, it may be a potential molecular target for enhanced amylose content in grain.
date: 2022-12-23
volume: 23
issue: 1
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Springer Science and Business Media LLC
type: Organization

Funct. Integr. Genomics
type: Collection

Kousar Jahan
type: Person
given name: Kousar
family name: Jahan

Vishnu K Sharma
type: Person
given name: Vishnu K
family name: Sharma

Vikas Rishi
type: Person
given name: Vikas
family name: Rishi

P V Bharatam
type: Person
given name: P V
family name: Bharatam

Vinita Sharma
type: Person
given name: Vinita
family name: Sharma

Anuradhika Puri
type: Person
given name: Anuradhika
family name: Puri

Deepak Sharma
type: Person
given name: Deepak
family name: Sharma

Prashant Kumar
type: Person
given name: Prashant
family name: Kumar

Ankita Mishra
type: Person
given name: Ankita
family name: Mishra

Joy Roy
type: Person
given name: Joy
family name: Roy

HTML Summary of #3392 Mechanistic insights into granule-bound starch synthase I (GBSSI.L539P) allele in high amylose starch biosynthesis in wheat (Triticum aestivum L.)
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primary topic: Mechanistic insights into granule-bound starch synthase I (GBSSI.L539P) allele in high amylose starch biosynthesis in wheat (Triticum aestivum L.)

QR Microbiology
type: Concept