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primary topic: Dual immunization with CdtB protein and flagellin epitope offers augmented protection against enteric fever in mice

Dual immunization with CdtB protein and flagellin epitope offers augmented protection against enteric fever in mice
type: AcademicArticle
type: Article
abstract: AIMS: Present study has explored the protective response of dual immunization using two different antigenic entities (i.e. flagellin epitope and cytolethal distending toxin subunit B (CdtB) protein) against lethal challenge of typhoidal serovars in a murine model. MAIN METHODS: In-vitro immunogenicity of flagellin epitope-BSA conjugate and CdtB protein was confirmed using Indirect ELISA of typhoid positive patients' sera. Further, both entities were administered intraperitoneally in mice individually or in combination, followed by lethal challenge of typhoidal Salmonellae. Various parameters were analysed such as bacterial burden, mice survival, histopathological analysis, cytokine analysis and immunophenotyping. Serum samples obtained from the immunized mice were used for passive immunization studies, wherein mice survival and mechanism of action of the generated antibodies was studied. KEY FINDINGS: Active immunization studies using the combination of both entities demonstrated improved mice survival after lethal challenge with typhoidal Salmonellae, reduced bacterial burden in organs, expression of immunophenotypic markers in splenocytes and restored tissue histoarchitecture. When used in combination, the effective doses of both the candidates reduced which may be attributed to multiprong approach used by the immune system to recognize Salmonella. Passive immunization studies further determined the protective efficacy of generated antibodies by different mechanisms such as complement mediated bactericidal action, swarming inhibition and increased phagocytic uptake. SIGNIFICANCE: Present study is the first phase of the proof-of-concept which may prove to be beneficial in developing an effective bi-functional vaccine candidate to render protection against both Vi-positive as well as Vi-negative Salmonella strains.
date: 2023-12-01
volume: 334
issue: 122216
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Elsevier BV
type: Organization

Life Sci.
type: Collection

Preeti Pathania
type: Person
given name: Preeti
family name: Pathania

Raghwendra Pratap Singh
type: Person
given name: Raghwendra Pratap
family name: Singh

Reena Thakur
type: Person
given name: Reena
family name: Thakur

Praveen Rishi
type: Person
given name: Praveen
family name: Rishi

Chander Raman Suri
type: Person
given name: Chander Raman
family name: Suri

Varsha Gupta
type: Person
given name: Varsha
family name: Gupta

Rashmi Kumar
type: Person
given name: Rashmi
family name: Kumar

Shania Vij
type: Person
given name: Shania
family name: Vij

HTML Summary of #3412 Dual immunization with CdtB protein and flagellin epitope offers augmented protection against enteric fever in mice
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primary topic: Dual immunization with CdtB protein and flagellin epitope offers augmented protection against enteric fever in mice

QR Microbiology
type: Concept