creators_name: Jyot, J
creators_name: Gautam, J K
creators_name: Raje, Manoj
creators_name: Ghosh, A
type: article
datestamp: 2012-01-03 16:50:27
lastmod: 2015-01-07 05:58:16
metadata_visibility: show
title: Localization of DnaK and GroEL in Vibrio cholerae.
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Vibrio cholerae; DnaK; GroEL; Localization
note: Copyright of this article belongs to Elsevier Science
abstract: Though the GroEL and DnaK heat shock proteins are well characterized in prokaryotes, only scanty and controversial information exist about their cellular localization. In the present study, the localization of the heat shock proteins DnaK and GroEL in normal and heat shocked cells of Vibrio cholerae, was investigated both by immunogold labeling of ultrathin sections and biochemical methods. Much of the DnaK was found to be localized at the inner membrane in unstressed cells, most probably at the Bayer's adhesion sites. Data suggested that upon heat shock, the DnaK associated with the membrane continued to remain there, but the newly synthesized DnaK appeared mostly in the cytoplasm. GroEL in both stressed and unstressed cells was found mainly in the cytoplasm.
date: 1999-03-15
date_type: published
publication: FEMS microbiology letters
volume: 172
number: 2
publisher: Elsevier Science
pagerange: 165-71
refereed: TRUE
issn: 0378-1097
official_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1999.tb13465.x/pdf
related_url_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1999.tb13465.x/pdf
related_url_type: pub
citation:   Jyot, J and Gautam, J K and Raje, Manoj and Ghosh, A  (1999) Localization of DnaK and GroEL in Vibrio cholerae.  FEMS microbiology letters, 172 (2).  pp. 165-71.  ISSN 0378-1097     
document_url: http://crdd.osdd.net/open/345/1/ghosh99.pdf