<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Heterologous expression, purification and functional characterization of recombinant serratiopeptidase from Serratia marcescens AD-W2"^^ . "Serratiopeptidase, a proteolytic enzyme with therapeutic applications, is traditionally produced from the bacterium Serratia marcescens. Recombinant production of serratiopeptidase in Escherichia coli offers a safer alternative to the biosafety concerns of the producer. Present study involves cloning and heterologous expression of thermoactive serratiopeptidase gene from S. marcescens AD-W2 in E. coli K12 in pET28a plasmid. Optimized expression conditions i.e. 37 degrees C, OD600 5, 5% L-rhamnose, 5mM IPTG, and 50% dissolved oxygen led to the final yield of 190 mg/g serratiopeptidase (4747 mg protein/L within 9 h in a bioreactor. Purification and refolding of recombinant serratiopeptidase was performed in a single step using Tangential Flow Filtration (TFF) and diafiltration process. The purified recombinant serratiopeptidase exhibited specific activity of 1800 Units/mg protein, with an optimal activity at pH 9.0 and temperature 50 degrees C. The value of kinetic constant Km was calculated as 1.38 mg/mL for casein. The recombinant serratiopeptidase demonstrated comparable anti-inflammatory activity to the commercially available serratiopeptidase, inhibiting nitric oxide release and pro-inflammatory cytokine production in LPS-stimulated murine macrophage cell line RAW 264.7. The study reveals that the recombinant serratiopeptidase produced in E. coli K12 holds promising source of safe and effective anti-inflammatory agent."^^ . "2026-02-24" . . "42" . "3" . . "SPRINGER"^^ . . . "WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY"^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Ravi P. N."^^ . "Mishra"^^ . "Ravi P. N. Mishra"^^ . . "Ravi S."^^ . "Manhas"^^ . "Ravi S. Manhas"^^ . . "Zabeer"^^ . "Ahmed"^^ . "Zabeer Ahmed"^^ . . "Diljeet"^^ . "Kumar"^^ . "Diljeet Kumar"^^ . . "Shubham"^^ . "Singh"^^ . "Shubham Singh"^^ . . "Asha"^^ . "Chaubey"^^ . "Asha Chaubey"^^ . . "Sanket"^^ . "Shukla"^^ . "Sanket Shukla"^^ . . "Diksha"^^ . "Koul"^^ . "Diksha Koul"^^ . . "Devtulya"^^ . "Chander"^^ . "Devtulya Chander"^^ . . . . . . "Heterologous expression, purification and functional characterization of recombinant serratiopeptidase from Serratia marcescens AD-W2 (HTML)"^^ . . . "references.bib"^^ . . "HTML Summary of #3465 \n\nHeterologous expression, purification and functional characterization of recombinant serratiopeptidase from Serratia marcescens AD-W2\n\n" . "text/html" . . . "QR Microbiology"@en . .