TY  - JOUR
CY  - VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
N1  - Copyright of this data belongs to WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ID  - open3465
UR  - https://link.springer.com/article/10.1007/s11274-026-04795-3
IS  - 3
A1  - Chander, Devtulya
A1  - Koul, Diksha
A1  - Singh, Shubham
A1  - Manhas, Ravi S.
A1  - Kumar, Diljeet
A1  - Shukla, Sanket
A1  - Mishra, Ravi P. N.
A1  - Ahmed, Zabeer
A1  - Chaubey, Asha
Y1  - 2026/02/24/
N2  - Serratiopeptidase, a proteolytic enzyme with therapeutic applications, is traditionally produced from the bacterium Serratia marcescens. Recombinant production of serratiopeptidase in Escherichia coli offers a safer alternative to the biosafety concerns of the producer. Present study involves cloning and heterologous expression of thermoactive serratiopeptidase gene from S. marcescens AD-W2 in E. coli K12 in pET28a plasmid. Optimized expression conditions i.e. 37 degrees C, OD600 5, 5% L-rhamnose, 5mM IPTG, and 50% dissolved oxygen led to the final yield of 190 mg/g serratiopeptidase (4747 mg protein/L within 9 h in a bioreactor. Purification and refolding of recombinant serratiopeptidase was performed in a single step using Tangential Flow Filtration (TFF) and diafiltration process. The purified recombinant serratiopeptidase exhibited specific activity of 1800 Units/mg protein, with an optimal activity at pH 9.0 and temperature 50 degrees C. The value of kinetic constant Km was calculated as 1.38 mg/mL for casein. The recombinant serratiopeptidase demonstrated comparable anti-inflammatory activity to the commercially available serratiopeptidase, inhibiting nitric oxide release and pro-inflammatory cytokine production in LPS-stimulated murine macrophage cell line RAW 264.7. The study reveals that the recombinant serratiopeptidase produced in E. coli K12 holds promising source of safe and effective anti-inflammatory agent.
PB  - SPRINGER
JF  - WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
VL  - 42
KW  - <italic>E.coli</italic> K12; serratiopeptidase; Protein refolding; Tangential flow filtration; Anti-inflammatory activity
TI  - Heterologous expression, purification and functional characterization of recombinant serratiopeptidase from Serratia marcescens AD-W2
ER  -