openIsolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)MumtazMumtazauthorSimranKaurauthorSimranGagnejaauthorVinod D.ChaudhariauthorRavi P. N.MishraauthorThe increasing prevalence of antibiotic-resistant infections necessitates the development of novel antimicrobial medications. Actinomycetes are recognized for producing a diverse array of naturally occurring biologically active compounds. Natural environments represent vast, largely untapped reservoirs of microbial diversity, harboring countless unexamined and unidentified microorganisms. During the antimicrobial screening in this study, we isolated an actinomycete strain named DGM-81 from sewage samples of a landfill. The morphological, biochemical, 16S rRNA gene sequence, and phylogenetic analysis show that DGM-81 resembles the actinomycete Gordonia paraffinivorans NBRC108238. Whole genome analysis revealed the presence of 4043 CDS (coding sequences) and a GC content of 69.16%. Pan genomic analysis signifies that this strain has an open pangenome, reflecting high genetic diversity. This suggests strong adaptive potential and frequent gene acquisition within the species. The antiSMASH tool was utilised to depict the biosynthetic clusters for secondary metabolic products, indicating that many clusters possess the signatures to produce bioactive compounds that remain unexplored. The ethyl acetate extract from the DGM-81 culture supernatant underwent purification via Flash chromatography and HPLC, resulting in a purified peak that exhibited antimicrobial activity against Methicillin-Resistant S. aureus. The purified fraction's NMR analysis divulged Daidzein, a compound hitherto unknown in this species. Furthermore, checkerboard assay demonstrated synergistic interactions between the purified compound and selected conventional antibiotics against MRSA. To the best of our knowledge, this is the first report on the antimicrobial activity of Gordonia paraffinivorans. Our findings demonstrate that metabolites derived from this bacterium exhibit significant inhibitory effects against a range of gram-positive pathogens including MRSA, suggesting its potential as a novel source of antimicrobial compounds, highlighting a wide range of possible industrial uses.QR Microbiology2026-03-24SPRINGERArticle

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