…
comment: The repository administrator has not yet configured an RDF license.
primary topic: Isolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)

Isolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)
type: AcademicArticle
type: Article
abstract: The increasing prevalence of antibiotic-resistant infections necessitates the development of novel antimicrobial medications. Actinomycetes are recognized for producing a diverse array of naturally occurring biologically active compounds. Natural environments represent vast, largely untapped reservoirs of microbial diversity, harboring countless unexamined and unidentified microorganisms. During the antimicrobial screening in this study, we isolated an actinomycete strain named DGM-81 from sewage samples of a landfill. The morphological, biochemical, 16S rRNA gene sequence, and phylogenetic analysis show that DGM-81 resembles the actinomycete Gordonia paraffinivorans NBRC108238. Whole genome analysis revealed the presence of 4043 CDS (coding sequences) and a GC content of 69.16%. Pan genomic analysis signifies that this strain has an open pangenome, reflecting high genetic diversity. This suggests strong adaptive potential and frequent gene acquisition within the species. The antiSMASH tool was utilised to depict the biosynthetic clusters for secondary metabolic products, indicating that many clusters possess the signatures to produce bioactive compounds that remain unexplored. The ethyl acetate extract from the DGM-81 culture supernatant underwent purification via Flash chromatography and HPLC, resulting in a purified peak that exhibited antimicrobial activity against Methicillin-Resistant S. aureus. The purified fraction's NMR analysis divulged Daidzein, a compound hitherto unknown in this species. Furthermore, checkerboard assay demonstrated synergistic interactions between the purified compound and selected conventional antibiotics against MRSA. To the best of our knowledge, this is the first report on the antimicrobial activity of Gordonia paraffinivorans. Our findings demonstrate that metabolites derived from this bacterium exhibit significant inhibitory effects against a range of gram-positive pathogens including MRSA, suggesting its potential as a novel sour…
date: 2026-03-24
volume: 83
issue: 5
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Isolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA) (HTML)
subject: QR Microbiology

Isolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA) (HTML)
type: Document
has file: references (1).bib
has part: references (1).bib

SPRINGER
type: Organization

CURRENT MICROBIOLOGY
type: Collection

Simran Kaur
type: Person
given name: Simran
family name: Kaur

Ravi P. N. Mishra
type: Person
given name: Ravi P. N.
family name: Mishra

Vinod D. Chaudhari
type: Person
given name: Vinod D.
family name: Chaudhari

Mumtaz Mumtaz
type: Person
given name: Mumtaz
family name: Mumtaz

Simran Gagneja
type: Person
given name: Simran
family name: Gagneja

HTML Summary of #3467 Isolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)
format: text/html
primary topic: Isolation, Screening, and Characterization of Gordonia paraffinivorans DGM-81 Exhibiting Antimicrobial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)

QR Microbiology
type: Concept