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primary topic: Comparative Genomics Analysis Reveals the Strain-specific Probiotic Potential and Putative Antimicrobial Peptides of Bifidobacterium breve NCIM 5671

Comparative Genomics Analysis Reveals the Strain-specific Probiotic Potential and Putative Antimicrobial Peptides of Bifidobacterium breve NCIM 5671
type: AcademicArticle
type: Article
abstract: Bifidobacterium breve is a dominant member of the infant gut microbiota and is widely recognized for its probiotic attributes, including carbohydrate metabolism, stress tolerance, and pathogen inhibition. In this study, we sequenced the whole genome of B. breve NCIM 5671 to assess its metabolic potential, adaptive traits, safety, and antimicrobial activity. We assembled the genome into 69 contigs totaling 2.41 Mb, with a GC content of 58.5%, and it encodes 2,189 genes. Functional annotation revealed a high abundance of genes involved in amino acid and carbohydrate metabolism. Pan-genome analysis of 50 B. breve genomes revealed an open pan-genome structure with 976 core genes and significant accessory and strain-specific gene diversity. Phylogenetic and ANI analyses placed NCIM 5671 within a clade of infant-derived strains. Notably, we identified multiple antimicrobial peptide biosynthetic gene clusters, including lanthipeptide and lasso peptide systems, some of which were previously reported in B. breve but remain functionally uncharacterized. In vitro analysis confirmed the production of a low-molecular-weight peptide with broad-spectrum antimicrobial activity, specifically inhibiting RNA biosynthesis, consistent with the known mechanism of lasso peptides. Safety assessment revealed no functional antibiotic resistance or virulence genes. Overall, this study establishes an integrated genomic and functional framework for elucidating strain-specific probiotic traits in NCIM 5671. Importantly, this is the first study to functionally explore a putative lasso peptide-associated antimicrobial system in B. breve.
date: 2026-05-19
volume: 83
issue: 7
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Comparative Genomics Analysis Reveals the Strain-specific Probiotic Potential and Putative Antimicrobial Peptides of Bifidobacterium breve NCIM 5671 (HTML)
subject: QR Microbiology

Comparative Genomics Analysis Reveals the Strain-specific Probiotic Potential and Putative Antimicrobial Peptides of Bifidobacterium breve NCIM 5671 (HTML)
type: Document
has file: references (2).bib
has part: references (2).bib

SPRINGER
type: Organization

CURRENT MICROBIOLOGY
type: Collection

Prakash M. Halami
type: Person
given name: Prakash M.
family name: Halami

Kusuma N. Puttaswamy
type: Person
given name: Kusuma N.
family name: Puttaswamy

Prabhu Patil
type: Person
given name: Prabhu
family name: Patil

Kanika Bansal
type: Person
given name: Kanika
family name: Bansal

Steji Raphel
type: Person
given name: Steji
family name: Raphel

Aravind Sundararaman
type: Person
given name: Aravind
family name: Sundararaman

HTML Summary of #3468 Comparative Genomics Analysis Reveals the Strain-specific Probiotic Potential and Putative Antimicrobial Peptides of Bifidobacterium breve NCIM 5671
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primary topic: Comparative Genomics Analysis Reveals the Strain-specific Probiotic Potential and Putative Antimicrobial Peptides of Bifidobacterium breve NCIM 5671

QR Microbiology
type: Concept