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primary topic: Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers

Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers
type: AcademicArticle
type: Article
abstract: Cardiac troponin I (cTnI) is a key biomarker for acute myocardial infarction (AMI); however, the available detection methods are limited by slow responses, complex steps, and costly instrumentation, restricting their suitability for rapid or point-of-care testing. Herein, we report a rapid and sensitive colorimetric assay for the detection of cTnI based on a nanoparticle shielding layer and its interfacial interactions with an enzyme. To achieve high sensitivity, we employed a dual-affinity silver nanoparticle (AgNP) probe coated with two highly specific affinity molecules, i.e., aptamer (Apt) and antibody (Ab), offering effective detection of the target molecule. The affinity layer coating was inherently imperfect, leaving exposed regions at the interface of the AgNP probe to inhibit the enzyme (urease) by Ag-SH interaction. Upon introduction of cTnI, the probe selectively binds to it, and a target-induced shielding layer is formed that prevents the interaction of the enzyme with the AgNP surface, rendering the enzyme active in solution. Consequently, the active enzyme generates a colored endpoint for visual detection in <15 min with a detection limit of 0.088 ng/mL. We assessed the assay performance in a complex matrix to evaluate the stringency. The working of the assay was studied using microscale thermophoresis (MST), which showed complete masking of the urease-particle interaction in the presence of cTnI. Unlike surface-based methods, such as ELISA, our solution-phase assay eliminates immobilization and blocking steps and achieves enhanced sensitivity, representing a paradigm shift in biomarker detection.
date: 2026-03-18
volume: 18
issue: 8
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers (HTML)
subject: QR Microbiology

Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers (HTML)
type: Document
has file: references (3).bib
has part: references (3).bib

AMER CHEMICAL SOC
type: Organization

ACS APPLIED MATERIALS & INTERFACES
type: Collection

Jagtar Singh
type: Person
given name: Jagtar
family name: Singh

Bala Bharti
type: Person
given name: Bala
family name: Bharti

Priya Bhardwaj
type: Person
given name: Priya
family name: Bhardwaj

S Sagrika
type: Person
given name: S
family name: Sagrika

Rajni Bala
type: Person
given name: Rajni
family name: Bala

Vijayender Bhalla
type: Person
given name: Vijayender
family name: Bhalla

Manojkumar Rohit
type: Person
given name: Manojkumar
family name: Rohit

Bhawana Bisht
type: Person
given name: Bhawana
family name: Bisht

HTML Summary of #3470 Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers
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primary topic: Target-Induced Shielding Layer at the Dual-Affinity Probe Interface for Rapid Colorimetric Detection of Cardiac Biomarkers

QR Microbiology
type: Concept