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primary topic: Poly(I:C) and R848 adjuvants elicit sizeable humoral immunity to liver stage malaria antigens

Poly(I:C) and R848 adjuvants elicit sizeable humoral immunity to liver stage malaria antigens
type: AcademicArticle
type: Article
abstract: Background and objectives: Plasmodium falciparum infection remains a major global health burden due to increasing drug resistance and lack of an effective vaccine. Targeting the exoerythrocytic liver stage (LS) of malaria infection offers a promising approach to achieve sterile immunity to malaria infection. Therefore, the present work investigates the immunogenic potential of two key LS malaria antigens: circumsporozoite protein of P. falciparum (PfCSP) and liver stage-expressed tryptophan-rich protein of P. berghei (PbSLTRiP) formulated with a dual toll-like receptor (TLR) adjuvant combination, Poly(I:C) and R848, to evaluate the potency and durability of the elicited humoral response. Methods: BALB/c and C57BL/6 mice were immunized using a multidose regimen with PfCSP and PbSLTRiP, either alone or in combination (Poly(I:C) and R848). Humoral immune responses were quantified 30-80 days post-immunization through total IgG kinetics, functional IgG isotypes (IgG1, IgG2c/IgG2a), and antibody avidity (AI). Results: Coadministration of Poly(I:C) and R848 was shown to evoke greater antibody responses compared to antigen alone. PfCSP-specific IgG titers remained detectable up to 80 days post immunization and declined gradually over time. PbSLTRiP immunization, however, produced progressive increases in total IgG, detectable IgG2c responses, and modest changes in antibody avidity, with little non-significant difference between adjuvant regimens. Conclusions: Combination of Poly(I:C) and R848 adjuvant induced detectable antibody responses against LS malaria antigens, with magnitudes generally like those obtained with single adjuvants. Limited statistical differences highlight preliminary trends warranting future investigations into predicted multi-epitope engagement and hypothesized B cell-mediated CD8+ T-cell cross-presentation.
date: 2026-03
volume: 17
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

FRONTIERS MEDIA
type: Organization

FRONTIERS IN IMMUNOLOGY
type: Collection

Agam P. Singh
type: Person
given name: Agam P.
family name: Singh

Sarat K. Dalai
type: Person
given name: Sarat K.
family name: Dalai

Devangkumar D. Trivedi
type: Person
given name: Devangkumar D.
family name: Trivedi

Mansi Thakkar
type: Person
given name: Mansi
family name: Thakkar

Rajeev K. Tyagi
type: Person
given name: Rajeev K.
family name: Tyagi

Sylviane Pied
type: Person
given name: Sylviane
family name: Pied

Mansi Ahuja
type: Person
given name: Mansi
family name: Ahuja

Chirag Prajapati
type: Person
given name: Chirag
family name: Prajapati

Jagrut Shah
type: Person
given name: Jagrut
family name: Shah

Nikunj Tandel
type: Person
given name: Nikunj
family name: Tandel

HTML Summary of #3475 Poly(I:C) and R848 adjuvants elicit sizeable humoral immunity to liver stage malaria antigens
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primary topic: Poly(I:C) and R848 adjuvants elicit sizeable humoral immunity to liver stage malaria antigens

QR Microbiology
type: Concept