creators_name: Banerjee, S K
creators_name: Bhatt, K
creators_name: Rana, S
creators_name: Misra, P
creators_name: Chakraborti, Pradip K
type: article
datestamp: 2011-12-13 16:45:06
lastmod: 2012-03-29 07:25:18
metadata_visibility: show
title: Involvement of an efflux system in mediating high level of fluoroquinolone resistance in Mycobacterium smegmatis.
ispublished: pub
subjects: QD
full_text_status: none
note: Copyright © 1996 Academic Press. All rights reserved.
abstract: A wild type strain of Mycobacterium smegmatis mc2 155 was serially adapted to 64 fold of minimal inhibitory concentration of an antimycobacterial agent, ciprofloxacin. This clone (CIPr) exhibited cross resistance to ofloxacin and ethidium bromide. The rate of drug efflux was accelerated in CIPr compared to the wild type strain. Verapamil, a calcium channel blocker, enhanced the drug accumulation in CIPr by diminishing the efflux and thus reversed the resistant phenotype. Additionally, a missense mutation was detected in the quinolone resistance determining region of the DNA-gyrase A subunit of CIPr. Taken together, these results suggest that drug efflux plays a major role in conferring such a high level of resistance in CIPr, in addition to the mutation in the DNA-gyrase locus.
date: 1996-09-13
date_type: published
publication: Biochemical and biophysical research communications
volume: 226
number: 2
publisher: Elsevier Science/Academic Press
pagerange: 362-8
refereed: TRUE
issn: 0006-291X
official_url: http://www.sciencedirect.com/science/article/pii/S0006291X96913625
related_url_url: http://www.sciencedirect.com/science/article/pii/S0006291X96913625
related_url_type: author
citation:   Banerjee, S K and Bhatt, K and Rana, S and Misra, P and Chakraborti, Pradip K  (1996) Involvement of an efflux system in mediating high level of fluoroquinolone resistance in Mycobacterium smegmatis.  Biochemical and biophysical research communications, 226 (2).  pp. 362-8.  ISSN 0006-291X