creators_name: Vinay, D S
creators_name: Raje, Manoj
creators_name: Mishra, G C
type: article
datestamp: 2011-12-13 16:47:47
lastmod: 2015-01-07 06:04:53
metadata_visibility: show
title: Characterization of a novel co-stimulatory molecule: a 155-160kD B cell surface protein provides accessory help to CD4+ T cells to proliferate and differentiate.
ispublished: pub
subjects: QR180
full_text_status: none
keywords: CD4+ T cells; B1; co-stimulation; liposomes
note: Copyright of this article belongs Elsevier Science
abstract: Optimal activation of T cells to clonally expand requires at least two distinct biological signals; one is generated by the interaction of the T cell receptor (TcR) with peptides bound to MHC molecules. The other signal(s) is (are) generated by a functionally defined event called the co-stimulatory pathway. We have characterized the co-stimulatory property of a murine B lymphocyte membrane protein (155-160 kD) on resting CD4+ T cells. The study involved the isolation of a 155-160 kD protein (B1) from the membranes of LPS-stimulated B cells. When reconstituted into lipid vesicles, B1 exerted a dose-dependent proliferative response to CD4+ T cells, resulting in the predominant secretion of IL-4 and IL-5 after cross-linking receptors with anti-CD3 mAb. This protein is a phosphoglycoprotein which gives a single spot on two-dimensional gel electrophoresis under reducing conditions and as a distinct peak on reverse phase-HPLC. The B1 binds to the T cell surface as is demonstrated by electron microscopic autoradiography and scanning electron microscopy, as well as competitive binding assays. It does not cross-react with antibodies directed against ICAM-1, LFA-1 alpha, B7, HSA and VCAM-1, suggesting the novelty of the protein. Activation of CD4+ T cells with B1 in the presence of anti-CD3 resulted in the translocation of protein kinase C (PKC). The B1 is barely detectable on the surface of resting B cells and digestion of this protein with V8 protease and peptide N-glycosidase F resulted in distinct protein bands on an autoradiogram.
date: 1996-01
date_type: published
publication: Molecular immunology
volume: 33
number: 1
pagerange: 1-14
refereed: TRUE
issn: 0161-5890
official_url: http://www.sciencedirect.com/science/article/pii/0161589095001336
related_url_url: http://www.sciencedirect.com/science/article/pii/0161589095001336
related_url_type: pub
citation:   Vinay, D S and Raje, Manoj and Mishra, G C  (1996) Characterization of a novel co-stimulatory molecule: a 155-160kD B cell surface protein provides accessory help to CD4+ T cells to proliferate and differentiate.  Molecular immunology, 33 (1).  pp. 1-14.  ISSN 0161-5890     
document_url: http://crdd.osdd.net/open/386/1/mishra1996.pdf