creators_name: Gupta, R
creators_name: Sharma, P
creators_name: Vyas, V V
type: article
datestamp: 2011-12-13 16:49:23
lastmod: 2011-12-13 16:49:23
metadata_visibility: show
title: Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli.
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Plasmid stability; Growth rate; E. coli; Environment
note: Copyright of this article belongs to Elsevier Science
abstract: The effect of environmental growth conditions on the segregational stability of a recombinant Escherichia coli-Bacillus subtilis shuttle plasmid pCPPS-31 which expresses carboxymethyl cellulase (CMCase) and contains the neomycin resistance gene (Ner), was studied in E. coli DH5 alpha. The stability increased with the decrease in medium complexity. Lowering culture temperatures had a negative effect on stability. Stability was maximum at 100 rpm and a medium/flask volume ratio of 1:10. A pH range of 5-8 had no significant effect on stability. The recombination (rec) and gyrase (gyr) backgrounds of E. coli hosts had no apparent effect on plasmid stability. The plasmid was structurally stable under all circumstances.
date: 1995-07-15
date_type: published
publication: Journal of biotechnology
volume: 41
number: 1
publisher: Elsevier Science
pagerange: 29-37
refereed: TRUE
issn: 0168-1656
official_url: http://www.sciencedirect.com/science/article/pii/016816569500049V
related_url_url: http://www.sciencedirect.com/science/article/pii/016816569500049V
related_url_type: pub
citation:   Gupta, R and Sharma, P and Vyas, V V  (1995) Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli.  Journal of biotechnology, 41 (1).  pp. 29-37.  ISSN 0168-1656     
document_url: http://crdd.osdd.net/open/394/1/vyas95.pdf