openMicromixing and the steady-state performance of bioreactors using recombinant bacteria--analysis through a reversed two-environment model.P RPatnaikauthorA reversed two-environment model has been used to study micromixing in continuous fermentation for growth and product formation by recombinant bacteria. As an example, an Escherichia coli M72 strain harbouring the plasmid pPLc23trpA1 and producing tryptophan synthetase is considered. With excess substrate, 10% plasmid-free bacteria in the initial broth do not affect the steady-state concentrations of plasmid-containing bacteria but their mass fractions decrease significantly. With a smaller substrate concentration, the mass fractions decrease sharply as the dilution rate increases if micromixing is good; but concentrations still remain high. There is also a clear demarcation between regions of good and poor micromixing. These results are explained and an optimal combination of micromixing and dilution rate is suggested to maximise productivity.QR Microbiology1994-12WileyArticle

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant open the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that open does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at open) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.