TY  - JOUR
N1  - Copyright of this article belongs to Wiley
ID  - open405
UR  - http://onlinelibrary.wiley.com/doi/10.1002/eji.1830240924/pdf
IS  - 9
A1  - Agrewala, J N
A1  - Vinay, D S
A1  - Joshi, A
A1  - Mishra, G C
Y1  - 1994/09//
N2  - In the present study, we describe the potential co-stimulatory role of a macrophage membrane-associated protein of 150 kDa (M150). The protein was isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was found to be a single molecule on two-dimensional gel electrophoresis. The molecule was re-constituted in phosphatidyl choline vesicles and tested for its ability to promote the proliferation and the secretion of lymphokines from T helper (Th) cells. The reconstituted M150 induced a significant proliferation of anti-CD3 monoclonal antibody (mAb)-stimulated ovalbumin-specific CD4+ T cells. Further, Th cells activated with this molecule in the presence of anti-CD3 mAb mainly secreted interleukin (IL)-2 and interferon-gamma but not IL-4. M150 could not promote the proliferation of Th cells, or lymphokine secretion in the absence of anti-CD3 mAb. These observations suggest that M150 acts by selectively activating a Th1-like immune response.
PB  - Wiley
JF  - European journal of immunology
VL  - 24
SN  - 0014-2980
TI  - A 150-kDa molecule of macrophage membrane stimulates interleukin-2 and interferon-gamma production and proliferation of ovalbumin-specific CD4+ T cells.
SP  - 2092
AV  - none
EP  - 7
ER  -