%0 Journal Article
%@ 0378-1097
%A Majumder, S
%A Gupta, R
%D 1994
%F open:406
%I Elsevier Science
%J FEMS microbiology letters
%K Mycobacterium microti; Interleukin-1 a; Mycobacteria; Cyclic AMP
%N 3
%P 329-34
%T Effect of interleukin-1 alpha on the growth of Mycobacterium microti within J774A.1 cells.
%U http://crdd.osdd.net/open/406/
%V 120
%X The effect of mouse recombinant interleukin-1 alpha on the intracellular growth of Mycobacterium microti in a murine macrophage cell line J774A.1 was investigated. Interleukin-1 alpha added after infection to the M. microti-infected macrophage monolayers enhanced the growth of M. microti in a concentration-dependent manner and this growth enhancement was abrogated by neutralization of interleukin-1 alpha with anti-interleukin-1 alpha antibody. Cyclic adenosine monophosphate level in J774A.1 cells was increased by the addition of interleukin-1 alpha. Addition of dibutyryl cyclic adenosine monophosphate to infected J774A.1 cells increased the number of intracellular bacteria in a concentration-dependent manner. These results suggest that interleukin-1 alpha acts as a growth enhancer for intracellular M. microti and the growth enhancing effect of interleukin-1 alpha may be due to enhanced cellular cyclic adenosine monophosphate level.
%Z Copyright of this article belongs to Elsevier Science