title: Novel assay for screening rifamycin B-producing mutants. creator: Vohra, R M subject: QR Microbiology description: A simple method that allows easy identification of rifamycin B-producing strains is described. This method involves the use of an enzyme, rifamycin oxidase, which converts inactive rifamycin B to active rifamycin S. In this method, colonies to be tested are grown in pairs. The two colonies are then transferred to two plates seeded with a sensitive strain of Staphylococcus aureus, one plate of which contains the enzyme rifamycin oxidase. All paired colonies which show a larger inhibition zone diameter on the enzyme-containing plate are identified as rifamycin B producers. publisher: ASM date: 1992-01 type: Article type: PeerReviewed format: application/pdf identifier: http://crdd.osdd.net/open/426/1/vohra92.pdf relation: http://aem.asm.org/content/58/1/403.full.pdf identifier: Vohra, R M (1992) Novel assay for screening rifamycin B-producing mutants. Applied and environmental microbiology, 58 (1). pp. 403-4. ISSN 0099-2240 relation: http://crdd.osdd.net/open/426/