title: Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.
creator: Sharma, Amit
creator: Radha Kishan, K V
subject: QR Microbiology
description: Protease inhibitors have been extensively used in research to prevent unwanted degradation of proteins during purification and analysis. Here, we report a remarkable discovery of protease inhibitor mediated reformation of peptide bonds by the serine protease inhibitor, PMSF in a diverse set of proteolyzed molecules. Interestingly, the religation reaction in the presence of PMSF occurs in a very short time period and with very high yields of the religated product. We also investigate the plausible mechanism of such a reaction and demonstrate through biochemical studies and X-ray crystallography that proximity of reacting termini is essential for the feasibility of this reaction.
publisher: Elsevier Science
date: 2011-11-04
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/445/1/radha11.pdf
relation: http://www.sciencedirect.com/science/article/pii/S0014579311007423
identifier:   Sharma, Amit and Radha Kishan, K V  (2011) Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.  FEBS letters, 585 (21).  pp. 3465-70.  ISSN 1873-3468     
relation: http://crdd.osdd.net/open/445/