creators_name: Arora, Pankaj Kumar
creators_name: Jain, R K
type: article
datestamp: 2011-12-09 14:12:56
lastmod: 2012-04-03 06:47:00
metadata_visibility: show
title: Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon.
ispublished: pub
subjects: QR
full_text_status: restricted
note: Copyright of this article belongs to Springer Science.
abstract: Degradation of 2-Chloro-4-nitrophenol (2C4NP) was studied by Arthrobacter sp. SJCon, isolated from the soil of a pesticide contaminated site. This strain utilized 2C4NP as sole source of carbon and energy and degraded 2C4NP with stoichiometric release of nitrite and chloride ions. A metabolite was detected during the study of 2C4NP degradation and identified as chlorohydroquinone (CHQ) by thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS). Inhibition study using 2,2'-dipyridyl showed that CHQ is a terminal aromatic compound in degradation pathway of 2C4NP. CHQ dioxygenase activity was observed in the crude extract of 2C4NP induced cells of the strain SJCon that suggested the cleavage of the CHQ to maleylacetate (MA). Our study clearly showed that Arthrobacter sp. SJCon degraded 2C4NP via formation of CHQ that further cleaved to MA by CHQ dioxygenase. This mechanism of degradation of 2C4NP differs from previously reported degradation pathways of 2C4NP.
date: 2011-12
date_type: published
publication: Current microbiology
volume: 63
number: 6
publisher: Springer Science
pagerange: 568-73
refereed: TRUE
issn: 1432-0991
official_url: http://www.springerlink.com/content/023k277u68786137/fulltext.pdf
related_url_url: http://www.springerlink.com/content/023k277u68786137/fulltext.pdf
related_url_type: pub
citation:   Arora, Pankaj Kumar and Jain, R K  (2011) Pathway for degradation of 2-chloro-4-nitrophenol in Arthrobacter sp. SJCon.  Current microbiology, 63 (6).  pp. 568-73.  ISSN 1432-0991     
document_url: http://crdd.osdd.net/open/447/1/arora11.1.pdf