creators_name: Pandey, Janmejay
creators_name: Heipieper, Hermann J
creators_name: Chauhan, Archana
creators_name: Arora, Pankaj Kumar
creators_name: Prakash, Dhan
creators_name: Takeo, Masahiro
creators_name: Jain, R K
type: article
datestamp: 2011-12-09 06:55:32
lastmod: 2015-01-09 11:14:38
metadata_visibility: show
title: Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98.
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Chlorinated nitroaromatic compounds .
Burkholderia . Aerobic degradation
note: Copyright of this article belongs to Springer Science.
abstract: Burkholderia sp. strain SJ98 (DSM 23195) was previously isolated and characterized for degradation and co-metabolic transformation of a number nitroaromatic compounds. In the present study, we evaluated its metabolic activity on chlorinated nitroaromatic compounds (CNACs). Results obtained during this study revealed that strain SJ98 can degrade 2-chloro-4-nitrophenol (2C4NP) and utilize it as sole source of carbon, nitrogen, and energy under aerobic conditions. The cells of strain SJ98 removed 2C4NP from the growth medium with sequential release of nearly stoichiometric amounts of chloride and nitrite in culture supernatant. Under aerobic degradation conditions, 2C4NP was transformed into the first intermediate that was identified as p-nitrophenol by high-performance liquid chromatography, LCMS-TOF, and GC-MS analyses. This transformation clearly establishes that the degradation of 2C4NP by strain SJ98 is initiated by "reductive dehalogenation"; an initiation mechanism that has not been previously reported for microbial degradation of CNAC under aerobic conditions.
date: 2011-11
date_type: published
publication: Applied microbiology and biotechnology
volume: 92
number: 3
publisher: Springer Science
pagerange: 597-607
refereed: TRUE
issn: 1432-0614
official_url: http://www.springerlink.com/content/p6378j3847216134/fulltext.pdf
related_url_url: http://www.springerlink.com/content/p6378j3847216134/fulltext.pdf
related_url_type: pub
citation:   Pandey, Janmejay and Heipieper, Hermann J and Chauhan, Archana and Arora, Pankaj Kumar and Prakash, Dhan and Takeo, Masahiro and Jain, R K  (2011) Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98.  Applied microbiology and biotechnology, 92 (3).  pp. 597-607.  ISSN 1432-0614     
document_url: http://crdd.osdd.net/open/466/1/jain11.pdf