TY  - JOUR
N1  - Copyright of this article belongs to Elsevier Science
ID  - open470
UR  - http://www.sciencedirect.com/science/article/pii/S0956566311001758
IS  - 10
A1  - Sharma, Priyanka
A1  - Sablok, Kavita
A1  - Bhalla, Vijayender
A1  - Suri, C Raman
Y1  - 2011/06/15/
N2  - A disposable electrochemical immunosensor has been developed for the determination of phenyl urea herbicide-diuron using a low cost laser ablated gold electrodes (LC-LAGE) fabricated on polystyrene substrate. The electrodes were electrochemically deposited with prussian blue-gold nanoparticle (PB-GNP) film, and a competitive inhibition immunoassay was performed on LC-LAGE by using a specific hapten-protein conjugate. The binding of available diuron specific antibody on conjugate coated electrode was detected using alkaline phosphatase rabbit anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of electrochemically active product, 1-naphthol, which was monitored using square wave voltammetry technique. The assay exhibited an excellent sensitivity and specificity showing the dynamic response range between 1 ppt and 10 ppm for diuron with detection limit around 1 ppt. This study provides insight into development of a rapid and high-throughput screening of pesticides in environmental samples at a very low cost.
PB  - Elsevier Science
JF  - Biosensors & bioelectronics
VL  - 26
SN  - 1873-4235
TI  - A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.
SP  - 4209
AV  - restricted
EP  - 12
ER  -