creators_name: Sharma, Priyanka
creators_name: Sablok, Kavita
creators_name: Bhalla, Vijayender
creators_name: Suri, C Raman
type: article
datestamp: 2011-12-09 06:56:33
lastmod: 2011-12-09 06:56:33
metadata_visibility: show
title: A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.
ispublished: pub
subjects: QD
full_text_status: restricted
note: Copyright of this article belongs to Elsevier Science
abstract: A disposable electrochemical immunosensor has been developed for the determination of phenyl urea herbicide-diuron using a low cost laser ablated gold electrodes (LC-LAGE) fabricated on polystyrene substrate. The electrodes were electrochemically deposited with prussian blue-gold nanoparticle (PB-GNP) film, and a competitive inhibition immunoassay was performed on LC-LAGE by using a specific hapten-protein conjugate. The binding of available diuron specific antibody on conjugate coated electrode was detected using alkaline phosphatase rabbit anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of electrochemically active product, 1-naphthol, which was monitored using square wave voltammetry technique. The assay exhibited an excellent sensitivity and specificity showing the dynamic response range between 1 ppt and 10 ppm for diuron with detection limit around 1 ppt. This study provides insight into development of a rapid and high-throughput screening of pesticides in environmental samples at a very low cost.
date: 2011-06-15
date_type: published
publication: Biosensors & bioelectronics
volume: 26
number: 10
publisher: Elsevier Science
pagerange: 4209-12
refereed: TRUE
issn: 1873-4235
official_url: http://www.sciencedirect.com/science/article/pii/S0956566311001758
related_url_url: http://www.sciencedirect.com/science/article/pii/S0956566311001758
related_url_type: pub
citation:   Sharma, Priyanka and Sablok, Kavita and Bhalla, Vijayender and Suri, C Raman  (2011) A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.  Biosensors & bioelectronics, 26 (10).  pp. 4209-12.  ISSN 1873-4235     
document_url: http://crdd.osdd.net/open/470/1/suri11.1.pdf