<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "N-Terminal sequencing by mass spectrometry through specific fluorescamine labeling of α-amino groups before tryptic digestion."^^ . "We present a single-step procedure for the specific mass labeling of unblocked protein N termini. We show that the dye fluorescamine, which is commonly assumed to require mildly alkaline conditions for undergoing a nonspecific reaction with α- and ε-amino groups associated with amino acids, in fact shows a specific reaction only with α-amino groups present at protein N termini when mildly acidic conditions are used. We use this finding to label, identify, and sequence the trypsinolysis-derived N-terminal peptide of lysozyme, using only mass spectrometry, to illustrate how this method could be used with other proteins."^^ . "2011-01-15" . "408" . "2" . . "Elsevier Science"^^ . . . "Analytical biochemistry"^^ . . . "10960309" . . . . . . . . . . . . . "Neeraj"^^ . "Dhaunta"^^ . "Neeraj Dhaunta"^^ . . "Purnananda"^^ . "Guptasarma"^^ . "Purnananda Guptasarma"^^ . . "Uzma"^^ . "Fatima"^^ . "Uzma Fatima"^^ . . . . . "HTML Summary of #495 \n\nN-Terminal sequencing by mass spectrometry through specific fluorescamine labeling of α-amino groups before tryptic digestion.\n\n" . "text/html" . . . "QD Chemistry"@en . .