TY  - JOUR
ID  - open495
UR  - http://www.sciencedirect.com/science/article/pii/S0003269710005853
IS  - 2
A1  - Dhaunta, Neeraj
A1  - Fatima, Uzma
A1  - Guptasarma, Purnananda
N2  - We present a single-step procedure for the specific mass labeling of unblocked protein N termini. We show that the dye fluorescamine, which is commonly assumed to require mildly alkaline conditions for undergoing a nonspecific reaction with ?- and ?-amino groups associated with amino acids, in fact shows a specific reaction only with ?-amino groups present at protein N termini when mildly acidic conditions are used. We use this finding to label, identify, and sequence the trypsinolysis-derived N-terminal peptide of lysozyme, using only mass spectrometry, to illustrate how this method could be used with other proteins.
VL  - 408
TI  - N-Terminal sequencing by mass spectrometry through specific fluorescamine labeling of ?-amino groups before tryptic digestion.
AV  - none
EP  - 8
N1  - Copyright of this article belongs to Elsevier Science
Y1  - 2011/01/15/
PB  - Elsevier Science
JF  - Analytical biochemistry
KW  -  Fluorescamine adduction; Mass spectrometry; Protein sequencing; N-terminal sequencing; pH effects; ?-Amino group reactivity


SN  - 1096-0309
SP  - 263
ER  -