@article{open503,
          volume = {33},
          number = {7},
           month = {November},
          author = {S Krishnamurthi and Arunachalam Ruckmani and R{\"u}diger Pukall and T Chakrabarti},
            note = {Copyright of this article belongs to Elsevier Science},
           title = {Psychrobacillus gen. nov. and proposal for reclassification of Bacillus insolitus Larkin \& Stokes, 1967, B. psychrotolerans Abd-El Rahman et al., 2002 and B. psychrodurans Abd-El Rahman et al., 2002 as Psychrobacillus insolitus comb. nov., Psychrobacillus psychrotolerans comb. nov. and Psychrobacillus psychrodurans comb. nov.},
       publisher = {Elsevier Science},
            year = {2010},
         journal = {Systematic and applied microbiology},
           pages = {367--73},
        keywords = {Bacillus rRNA group 2; 16S rRNA gene; FAME; Polar lipids; Ribotype

},
             url = {http://crdd.osdd.net/open/503/},
        abstract = {The taxonomic status of three Bacillus species, Bacillus insolitus, B. psychrodurans and B. psychrotolerans was reexamined using a polyphasic approach. In our analysis, these three Bacillus species formed a cluster separate from other members of Bacillus rRNA group 2 [5] and from Bacillus sensu stricto. These three species shared high 16S rRNA gene sequence similarities between them (97.8-99.7\%) and showed closest sequence similarity (95.3-96.3\%) to Paenisporosarcina quisquiliarum gen. nov., sp. nov. [18]. Sequence similarities with other related genera ranged between 90.9\% and 94.5\%. Phylogenetic coherence of the three species was supported by phenotypic characteristics, such as growth at low temperatures, negative oxidation and assimilation of many carbohydrates, MK8 as the major isoprenoid quinine and broadly similar polar lipid profiles. All three species had a similar peptidoglycan type of the variation A4{\ensuremath{\beta}} and similar genomic G+C contents (35.7-36.6 mol\% [1]). Genomic relatedness among them was shown to be less than 70\% and justified their separate species status [1]. These three species could be differentiated from each other and from related taxa on the basis of phenotypic, including chemotaxonomic, characteristics and ribotype patterns. On the basis of our analysis, we propose a new genus Psychrobacillus gen. nov. and to transfer B. insolitus, B. psychrodurans and B. psychrotolerans to the new genus as Psychrobacillus insolitus comb. nov. (type species of the genus; type strain W16B(T)=DSM 5(T)), P. psychrodurans comb. nov. (type strain 68E3(T)=DSM 11713(T)) and P. psychrotolerans comb. nov. (type strain 3H1(T)=DSM 11706(T)).}
}