title: Efficient biotransformation of herbicide diuron by bacterial strain Micrococcus sp. PS-1.
creator: Sharma, Priyanka
creator: Chopra, Adity
creator: Cameotra, Swaranjit Singh
creator: Suri, C Raman
subject: QR Microbiology
description: A Gram-positive, Micrococcus sp. strain PS-1 capable of utilizing phenylurea herbicide diuron as a sole carbon source at a high concentration (up to 250 ppm) was isolated from diuron storage site by selective enrichment study. The taxonomic characterization with 16S rRNA gene sequencing (1,477 bp) identified PS-1 as a member of Micrococcus sp. It was studied for the degradation of diuron and a range of its analogues (monuron, linuron, monolinuron, chlortoluron and fenuron). The shake flasks experiments demonstrated fast degradation of diuron (up to 96% at 250 ppm within 30 h incubation) with the addition of small quantity (0.01%) of non-ionic detergent. The relative degradation profile by the isolate was in the order of fenuron > monuron > diuron > linuron > monolinuron > chlortoluron. Further, the biochemical characterization of catabolic pathway by spectroscopic and chromatographic techniques demonstrated that the degradation proceeded via formation of dealkylated metabolites to form 3,4-dichloroaniline (3,4-DCA). It was the major metabolite formed, associated with profound increase in degradation kinetics in presence of appropriate additive.
publisher: Springer Science
date: 2010-11
type: Article
type: PeerReviewed
format: application/pdf
identifier: http://crdd.osdd.net/open/521/1/suri10.pdf
relation: http://www.springerlink.com/content/6812170070521675/fulltext.pdf
identifier:   Sharma, Priyanka and Chopra, Adity and Cameotra, Swaranjit Singh and Suri, C Raman  (2010) Efficient biotransformation of herbicide diuron by bacterial strain Micrococcus sp. PS-1.  Biodegradation, 21 (6).  pp. 979-87.  ISSN 1572-9729     
relation: http://crdd.osdd.net/open/521/