%0 Journal Article
%@ 1572-9729
%A Sharma, Priyanka
%A Chopra, Adity
%A Cameotra, Swaranjit Singh
%A Suri, C Raman
%D 2010
%F open:521
%I Springer Science
%J Biodegradation
%K Micrococcus sp.; Degradation;Phenylurea herbicides;Diuron 3,4-DCA
%N 6
%P 979-87
%T Efficient biotransformation of herbicide diuron by bacterial strain Micrococcus sp. PS-1.
%U http://crdd.osdd.net/open/521/
%V 21
%X A Gram-positive, Micrococcus sp. strain PS-1 capable of utilizing phenylurea herbicide diuron as a sole carbon source at a high concentration (up to 250 ppm) was isolated from diuron storage site by selective enrichment study. The taxonomic characterization with 16S rRNA gene sequencing (1,477 bp) identified PS-1 as a member of Micrococcus sp. It was studied for the degradation of diuron and a range of its analogues (monuron, linuron, monolinuron, chlortoluron and fenuron). The shake flasks experiments demonstrated fast degradation of diuron (up to 96% at 250 ppm within 30 h incubation) with the addition of small quantity (0.01%) of non-ionic detergent. The relative degradation profile by the isolate was in the order of fenuron > monuron > diuron > linuron > monolinuron > chlortoluron. Further, the biochemical characterization of catabolic pathway by spectroscopic and chromatographic techniques demonstrated that the degradation proceeded via formation of dealkylated metabolites to form 3,4-dichloroaniline (3,4-DCA). It was the major metabolite formed, associated with profound increase in degradation kinetics in presence of appropriate additive.
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