TY  - JOUR
ID  - open521
UR  - http://www.springerlink.com/content/6812170070521675/fulltext.pdf
IS  - 6
A1  - Sharma, Priyanka
A1  - Chopra, Adity
A1  - Cameotra, Swaranjit Singh
A1  - Suri, C Raman
N2  - A Gram-positive, Micrococcus sp. strain PS-1 capable of utilizing phenylurea herbicide diuron as a sole carbon source at a high concentration (up to 250 ppm) was isolated from diuron storage site by selective enrichment study. The taxonomic characterization with 16S rRNA gene sequencing (1,477 bp) identified PS-1 as a member of Micrococcus sp. It was studied for the degradation of diuron and a range of its analogues (monuron, linuron, monolinuron, chlortoluron and fenuron). The shake flasks experiments demonstrated fast degradation of diuron (up to 96% at 250 ppm within 30 h incubation) with the addition of small quantity (0.01%) of non-ionic detergent. The relative degradation profile by the isolate was in the order of fenuron > monuron > diuron > linuron > monolinuron > chlortoluron. Further, the biochemical characterization of catabolic pathway by spectroscopic and chromatographic techniques demonstrated that the degradation proceeded via formation of dealkylated metabolites to form 3,4-dichloroaniline (3,4-DCA). It was the major metabolite formed, associated with profound increase in degradation kinetics in presence of appropriate additive.
VL  - 21
TI  - Efficient biotransformation of herbicide diuron by bacterial strain Micrococcus sp. PS-1.
AV  - restricted
EP  - 87
N1  - Copyright of this article belongs Springer Science
Y1  - 2010/11//
PB  - Springer Science
JF  - Biodegradation
KW  - Micrococcus sp.; Degradation;Phenylurea herbicides;Diuron 3
KW  - 4-DCA
SN  - 1572-9729
SP  - 979
ER  -