title: Efficient ligation and cloning of DNA fragments with 2-bp overhangs.
creator: Ranjan, Rajiv Kumar
creator: Rajagopal, Kammara
subject: QD Chemistry
description: Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers.
publisher: Elsevier Science
date: 2010-07-01
type: Article
type: PeerReviewed
relation: http://www.sciencedirect.com/science/article/pii/S0003269710001880
identifier:   Ranjan, Rajiv Kumar and Rajagopal, Kammara  (2010) Efficient ligation and cloning of DNA fragments with 2-bp overhangs.  Analytical biochemistry, 402 (1).  pp. 91-2.  ISSN 1096-0309     
relation: http://crdd.osdd.net/open/526/