<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Efficient ligation and cloning of DNA fragments with 2-bp overhangs."^^ . "Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers."^^ . "2010-07-01" . "402" . "1" . . "Elsevier Science"^^ . . . "Analytical biochemistry"^^ . . . "10960309" . . . . . . . . . . "Rajiv Kumar"^^ . "Ranjan"^^ . "Rajiv Kumar Ranjan"^^ . . "Kammara"^^ . "Rajagopal"^^ . "Kammara Rajagopal"^^ . . . . . "HTML Summary of #526 \n\nEfficient ligation and cloning of DNA fragments with 2-bp overhangs.\n\n" . "text/html" . . . "QD Chemistry"@en . .