TY  - JOUR
N1  - Copyright of this article belongs to Elsevier Science
ID  - open526
UR  - http://www.sciencedirect.com/science/article/pii/S0003269710001880
IS  - 1
A1  - Ranjan, Rajiv Kumar
A1  - Rajagopal, Kammara
Y1  - 2010/07/01/
N2  - Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning of DNA fragments with 2-bp overhangs. We present a simple method for the efficient ligation of DNA fragments with 2-bp overhanging ends, ranging in size from 0.7 to 2.5 kbp. Our method involves the initial heating and flash freezing of the vector-insert DNA mix, and a subsequent unique ligation reaction. This method provides a new molecular biology tool for researchers.
PB  - Elsevier Science
JF  - Analytical biochemistry
VL  - 402
SN  - 1096-0309
TI  - Efficient ligation and cloning of DNA fragments with 2-bp overhangs.
SP  - 91
AV  - none
EP  - 2
ER  -