<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Characterization of hapten-protein conjugates: antibody generation and immunoassay development for chlorophenoxyacetic acid pesticides."^^ . "The generation of specific and sensitive antibodies against small molecules is greatly dependent upon the characteristics of the hapten-protein conjugates. In this study, we report a new fluorescence-based method for the characterization of hapten-protein conjugates. The method is based on an effect promoted by hapten-protein conjugation density upon the fluorescence intensity of the intrinsic tryptophan chromophore molecules of the protein. The proposed methodology is applied to quantify the hapten-protein conjugation density for two different chlorophenoxyacetic acid pesticides, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenoxybutyric acid (2,4-DB), coupled to carrier protein. Highly sensitive anti-2,4-D and anti-2,4-DB antibodies were obtained using these well-characterized hapten-protein conjugates. The generated antibodies were used in an immunoassay format demonstrating inhibitory concentration (IC50) values equal to 30 and 7 ng/mL for 2,4-D and 2,4-DB, respectively. Linearity was observed in the concentration range between 0.1-500 nglmL with LODs around 4 and 3 ng/mL for 2,4-D and 2,4-DB, respectively, in standard water samples. The proposed method was successfully applied for the determination of the extent of hapten-protein conjugation to produce specific antibodies for immunoassay development against pesticides."^^ . "2009-12" . "92" . "6" . . "AOAC International"^^ . . . "Journal of AOAC International"^^ . . . "10603271" . . . . . . . . . . . . . "C Raman"^^ . "Suri"^^ . "C Raman Suri"^^ . . "Robin C"^^ . "Boro"^^ . "Robin C Boro"^^ . . "K Vikas"^^ . "Singh"^^ . "K Vikas Singh"^^ . . . . . "HTML Summary of #533 \n\nCharacterization of hapten-protein conjugates: antibody generation and immunoassay development for chlorophenoxyacetic acid pesticides.\n\n" . "text/html" . . . "QD Chemistry"@en . .