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primary topic: Critical role of RPI1 in the stress tolerance of yeast during ethanolic fermentation.

Critical role of RPI1 in the stress tolerance of yeast during ethanolic fermentation.
type: AcademicArticle
type: Article
abstract: Stress tolerance of yeast Saccharomyces cerevisiae during ethanolic fermentation is poorly understood due to the lack of genetic screens and conventional plate assays for studying this phenotype. We screened a genomic expression library of yeast to identify gene(s) that, upon overexpression, would prolong the survival of yeast cells during fermentation, with the view to understand the stress response better and to use the identified gene(s) in strain improvement. The yeast RPI1 (Ras-cAMP pathway inhibitor 1) gene was identified in such a screen performed at 38 degrees C; introducing an additional copy of RPI1 with its native promoter helped the cells to retain their viability by over 50-fold better than the wild type (WT) parent strain, after 36 h of fermentation at 38 degrees C. Disruption of RPI1 resulted in a drastic reduction in viability during fermentation, but not during normal growth, further confirming the role of this gene in fermentation stress tolerance. This gene seems to improve viability by fortifying the yeast cell wall, because RPI1 overexpression strain is highly resistant to cell lytic enzyme zymolyase, compared with the WT strain. As the RPI1 overexpression strain substantially retains cell viability at the end of fermentation, the cells can be reused in the subsequent round of fermentation, which is likely to facilitate economical production of ethanol.
date: 2009-12
volume: 9
issue: 8
is part of: FEMS yeast research
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
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subject: QR Microbiology

Critical role of RPI1 in the stress tolerance of yeast during ethanolic fermentation. (PDF)
type: Document

Blackwell Publishing/Wiley
type: Organization

FEMS yeast research
type: Collection
issn: 15671364

K Ganesan
type: Person
given name: K
family name: Ganesan

M Amin-ul Mannan
type: Person
given name: M Amin-ul
family name: Mannan

Rohini Chopra-Dewasthaly
type: Person
given name: Rohini
family name: Chopra-Dewasthaly

Rekha Puria
type: Person
given name: Rekha
family name: Puria

Critical role of RPI1 in the stress tolerance of yeast during ethanolic fermentation. (Image (PNG))
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primary topic: Critical role of RPI1 in the stress tolerance of yeast during ethanolic fermentation.

QR Microbiology
type: Concept