<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis."^^ . "Mycobacterium tuberculosis truncated hemoglobin, HbN, is endowed with a potent nitric-oxide dioxygenase activity and has been found to relieve nitrosative stress and enhance in vivo survival of a heterologous host, Salmonella enterica Typhimurium, within the macrophages. These findings implicate involvement of HbN in the defense of M. tuberculosis against nitrosative stress. The protein carries a tunnel system composed of a short and a long tunnel branch that has been proposed to facilitate diatomic ligand migration to the heme and an unusual Pre-A motif at the N terminus, which does not contribute significantly to the structural integrity of the protein, as it protrudes out of the compact globin fold. Strikingly, deletion of Pre-A region from the M. tuberculosis HbN drastically reduces its ability to scavenge nitric oxide (NO), whereas its insertion at the N terminus of Pre-A lacking HbN of Mycobacterium smegmatis improved its nitric-oxide dioxygenase activity. Titration of the oxygenated adduct of HbN and its mutants with NO indicated that the stoichiometric oxidation of protein is severalfold slower when the Pre-A region is deleted in HbN. Molecular dynamics simulations show that the excision of Pre-A motif results in distinct changes in the protein dynamics, which cause the gate of the tunnel long branch to be trapped into a closed conformation, thus impeding migration of diatomic ligands toward the heme active site. The present study, thus, unequivocally demonstrates vital function of Pre-A region in NO scavenging and unravels its unique role by which HbN might attain its efficient NO-detoxification ability."^^ . "2009-05-22" . . "284" . "21" . . "ASBMB"^^ . . . "The Journal of biological chemistry"^^ . . . "00219258" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "F Javier"^^ . "Luque"^^ . "F Javier Luque"^^ . . "Sudesh"^^ . "Pawaria"^^ . "Sudesh Pawaria"^^ . . "Kanak L"^^ . "Dikshit"^^ . "Kanak L Dikshit"^^ . . "Marcelo"^^ . "Martí"^^ . "Marcelo Martí"^^ . . "José Luis"^^ . "Gelpí"^^ . "José Luis Gelpí"^^ . . "Axel"^^ . "Bidon-Chanal"^^ . "Axel Bidon-Chanal"^^ . . "Arvind"^^ . "Anand"^^ . "Arvind Anand"^^ . . "Swati"^^ . "Arya"^^ . "Swati Arya"^^ . . "Dario A"^^ . "Estrin"^^ . "Dario A Estrin"^^ . . "Amrita"^^ . "Lama"^^ . "Amrita Lama"^^ . . . . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis. (PDF)"^^ . . . . . "dikshit09.pdf"^^ . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis. (Image (PNG))"^^ . . . . . . "lightbox.jpg"^^ . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis. (Image (PNG))"^^ . . . . . . "preview.jpg"^^ . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis. (Image (PNG))"^^ . . . . . . "medium.jpg"^^ . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis. (Image (PNG))"^^ . . . . . . "small.jpg"^^ . . . "Role of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis. (Indexer Terms)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #564 \n\nRole of Pre-A motif in nitric oxide scavenging by truncated hemoglobin, HbN, of Mycobacterium tuberculosis.\n\n" . "text/html" . . . "QD Chemistry"@en . .