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primary topic: Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii.

Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii.
type: AcademicArticle
type: Article
abstract: Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. The molecular mechanisms underlying its extreme osmotolerance and halotolerance have drawn considerable attention in the recent past. However, progress in this regard has been limited due to lack of availability of a transformation system and molecular tools to study the functions of the genes in D. hansenii. Here, we have described the development of an efficient transformation system for D. hansenii that is based on a histidine auxotrophic recipient strain and the DhHIS4 gene as the selectable marker. By screening the D. hansenii genomic library, we have isolated several autonomous replication sequences that can be used for constructing a replicating vector. Moreover, our study is the first to demonstrate gene disruption in D. hansenii by homologous recombination.
date: 2009-02
volume: 9
issue: 1
is part of: FEMS yeast research
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. (PDF)
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subject: QR Microbiology

Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. (PDF)
type: Document

W
type: Organization

FEMS yeast research
type: Collection
issn: 15671364

Anupriya Minhas
type: Person
given name: Anupriya
family name: Minhas

Dipanwita Biswas
type: Person
given name: Dipanwita
family name: Biswas

Alok K Mondal
type: Person
given name: Alok K
family name: Mondal

Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. (Image (PNG))
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Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. (Image (PNG))
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Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. (Image (PNG))
type: Document
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Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii. (Indexer Terms)
type: Document
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primary topic: Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii.

QR Microbiology
type: Concept