creators_name: Minhas, Anupriya
creators_name: Biswas, Dipanwita
creators_name: Mondal, Alok K
type: article
datestamp: 2011-12-08 19:36:35
lastmod: 2011-12-08 19:36:35
metadata_visibility: show
title: Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii.
ispublished: pub
subjects: QR
full_text_status: restricted
keywords: Debaryomyces hansenii;transformation;gene disruption;halotolerant yeast
note: Copyright of this article belongs to Wiley
abstract: Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. The molecular mechanisms underlying its extreme osmotolerance and halotolerance have drawn considerable attention in the recent past. However, progress in this regard has been limited due to lack of availability of a transformation system and molecular tools to study the functions of the genes in D. hansenii. Here, we have described the development of an efficient transformation system for D. hansenii that is based on a histidine auxotrophic recipient strain and the DhHIS4 gene as the selectable marker. By screening the D. hansenii genomic library, we have isolated several autonomous replication sequences that can be used for constructing a replicating vector. Moreover, our study is the first to demonstrate gene disruption in D. hansenii by homologous recombination.
date: 2009-02
date_type: published
publication: FEMS yeast research
volume: 9
number: 1
publisher: W
pagerange: 95-102
refereed: TRUE
issn: 1567-1364
official_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2008.00457.x/pdf
related_url_url: http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2008.00457.x/pdf
related_url_type: pub
citation:   Minhas, Anupriya and Biswas, Dipanwita and Mondal, Alok K  (2009) Development of host and vector for high-efficiency transformation and gene disruption in Debaryomyces hansenii.  FEMS yeast research, 9 (1).  pp. 95-102.  ISSN 1567-1364     
document_url: http://crdd.osdd.net/open/572/1/mandal09.pdf