TY  - JOUR
ID  - open584
UR  - http://ukpmc.ac.uk/abstract/MED/18662319/reload=0;jsessionid=0261A06122A9A663B09D872C1F5DA39A
IS  - 6
A1  - Thakur, Anil
A1  - Kaur, Jaspreet
A1  - Bachhawat, Anand K
N2  - The Schizosaccharomyces pombe ORF, SPAC29B12.10c, a predicted member of the oligopeptide transporter (OPT) family, was identified as a gene encoding the S. pombe glutathione transporter (Pgt1) by a genetic strategy that exploited the requirement of the cys1aDelta strain of S. pombe (which is defective in cysteine biosynthesis) for either cysteine or glutathione, for growth. Disruption of the ORF in the cys1aDelta strain led to an inability to grow on glutathione as a source of cysteine. Cloning and subsequent biochemical characterization of the ORF revealed that a high-affinity transporter for glutathione (K(m)=63 microM) that was found to be localized to the plasma membrane. The transporter was specific for glutathione, as significant inhibition in glutathione uptake could be observed only by either reduced or oxidized glutathione, or glutathione conjugates, but not by dipeptides or tripeptides. Furthermore, although glu-cys-gly, an analogue of glutathione (gamma-glu-cys-gly), could be utilized as a sulphur source, the growth was not Pgt1 dependent. This further underlined the specificity of this transporter for glutathione. The strong repression of pgt1(+) expression by cysteine suggested a role in scavenging glutathione from the extracellular environment for the maintenance of sulphur homeostasis in this yeast.
VL  - 8
TI  - Pgt1, a glutathione transporter from the fission yeast Schizosaccharomyces pombe.
AV  - restricted
EP  - 29
N1  - Copyright of this article belongs to Wiley
Y1  - 2008/09//
PB  - Wiley
JF  - FEMS yeast research
KW  - glutathione transporter;oligopeptide transporter family;sulphur metabolism;Schizosaccharomyces pombe
SN  - 1567-1356
SP  - 916
ER  -