creators_name: Kapoor, Divya
creators_name: Kumar, Vijay
creators_name: Chandrayan, Sanjeev Kumar
creators_name: Ahmed, Shubbir
creators_name: Sharma, Swati
creators_name: Datt, Manish
creators_name: Singh, Balvinder
creators_name: Karthikeyan, Subramanian
creators_name: Guptasarma, Purnananda
type: article
datestamp: 2011-12-08 19:38:15
lastmod: 2012-03-29 06:46:14
metadata_visibility: show
title: Replacement of the active surface of a thermophile protein by that of a homologous mesophile protein through structure-guided 'protein surface grafting'.
ispublished: pub
subjects: QR
full_text_status: restricted
note: Copyright of this article belongs to Elsevier Science.
abstract: Using several tens of rationally-selected substitutions, insertions and deletions of predominantly non-contiguous residues, we have remodeled the solvent-exposed face of a beta sheet functioning as the substrate-binding and catalytically-active groove of a thermophile cellulase (Rhodothermus marinus Cel12A) to cause it to resemble, both in its structure and function, the equivalent groove of a mesophile homolog (Trichoderma reesei Cel12A). The engineered protein, a mesoactive-thermostable cellulase (MT Cel12A) displays the temperature of optimal function of its mesophile ancestor and the temperature of melting of its thermophile ancestor, suggesting that such 'grafting' of a mesophile-derived surface onto a thermophile-derived structural scaffold can potentially help generate novel enzymes that recombine structural and functional features of homologous proteins sourced from different domains of life.
date: 2008-11
date_type: published
publication: Biochimica et biophysica acta
volume: 1784
number: 11
publisher: Elsevier Science
pagerange: 1771-6
refereed: TRUE
issn: 0006-3002
official_url: http://www.sciencedirect.com/science/article/pii/S1570963908001684
related_url_url: http://www.sciencedirect.com/science/article/pii/S1570963908001684
related_url_type: pub
citation:   Kapoor, Divya and Kumar, Vijay and Chandrayan, Sanjeev Kumar and Ahmed, Shubbir and Sharma, Swati and Datt, Manish and Singh, Balvinder and Karthikeyan, Subramanian and Guptasarma, Purnananda  (2008) Replacement of the active surface of a thermophile protein by that of a homologous mesophile protein through structure-guided 'protein surface grafting'.  Biochimica et biophysica acta, 1784 (11).  pp. 1771-6.  ISSN 0006-3002     
document_url: http://crdd.osdd.net/open/588/1/guptasarma08.16.pdf