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primary topic: Responses of Mycobacterium tuberculosis hemoglobin promoters to in vitro and in vivo growth conditions.

Responses of Mycobacterium tuberculosis hemoglobin promoters to in vitro and in vivo growth conditions.
type: AcademicArticle
type: Article
abstract: The success of Mycobacterium tuberculosis as one of the dreaded human pathogens lies in its ability to utilize different defense mechanisms in response to the varied environmental challenges during the course of its intracellular infection, latency, and reactivation cycle. Truncated hemoglobins trHbN and trHbO are thought to play pivotal roles in the cellular metabolism of this organism during stress and hypoxia. To delineate the genetic regulation of the M. tuberculosis hemoglobins, transcriptional fusions of the promoters of the glbN and glbO genes with green fluorescent protein were constructed, and their responses were monitored in Mycobacterium smegmatis and M. tuberculosis H37Ra exposed to environmental stresses in vitro and in M. tuberculosis H37Ra after in vivo growth inside macrophages. The glbN promoter activity increased substantially during stationary phase and was nearly 3- to 3.5-fold higher than the activity of the glbO promoter, which remained more or less constant during different growth phases in M. smegmatis, as well as in M. tuberculosis H37Ra. In both mycobacterial hosts, the glbN promoter activity was induced 1.5- to 2-fold by the general nitrosative stress inducer, nitrite, as well as the NO releaser, sodium nitroprusside (SNP). The glbO promoter was more responsive to nitrite than to SNP, although the overall increase in its activity was much less than that of the glbN promoter. Additionally, the glbN promoter remained insensitive to the oxidative stress generated by H(2)O(2), but the glbO promoter activity increased nearly 1.5-fold under similar conditions, suggesting that the trHb gene promoters are regulated differently under nitrosative and oxidative stress conditions. In contrast, transition metal-induced hypoxia enhanced the activity of both the glbN and glbO promoters at all growth phases; the glbO promoter was induced approximately 2.3-fold, which was found to be the highest value for this promoter under all the conditions evaluated. …
date: 2008-06
volume: 74
issue: 11
is part of: Applied and environmental microbiology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Responses of Mycobacterium tuberculosis hemoglobin promoters to in vitro and in vivo growth conditions. (PDF)
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subject: QR Microbiology

Responses of Mycobacterium tuberculosis hemoglobin promoters to in vitro and in vivo growth conditions. (PDF)
type: Document

AEM
type: Organization

Applied and environmental microbiology
type: Collection
issn: 10985336

Kanak L Dikshit
type: Person
given name: Kanak L
family name: Dikshit

Sudesh Pawaria
type: Person
given name: Sudesh
family name: Pawaria

Manoj Raje
type: Person
given name: Manoj
family name: Raje

Amrita Lama
type: Person
given name: Amrita
family name: Lama

Responses of Mycobacterium tuberculosis hemoglobin promoters to in vitro and in vivo growth conditions. (Indexer Terms)
type: Document
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primary topic: Responses of Mycobacterium tuberculosis hemoglobin promoters to in vitro and in vivo growth conditions.

QR Microbiology
type: Concept