creators_name: Bhasin, Manoj
creators_name: Lata, Sneh
creators_name: Raghava, G.P.S.
type: article
datestamp: 2012-01-10 08:06:00
lastmod: 2012-01-10 08:06:00
metadata_visibility: show
title: Searching and mapping of T-cell epitopes, MHC binders, and TAP binders.
ispublished: pub
subjects: QR180
full_text_status: restricted
keywords: database; MHC binders; TAP binders; T-cell epitopes
note: Copyright of this article belongs to Springer Science.
abstract: This chapter describes searching and mapping tools of MHCBN database, which is a curated database. It comprises over 23,000 peptide sequences, whose binding affinity with major histocompatibility complex (MHC) or transporter associated with antigen processing (TAP) molecules has been assayed experimentally. Each entry of the database provides full information (such as sequence, its MHC- or TAP-binding specificity, and source protein) about peptide whose binding affinity (IC50) and T-cell activity is experimentally determined. MHCBN has number of web-based tools for analyzing and retrieving information. In this chapter, we describe how to use web tools integrated in MHCBN that include (i) mapping of experimentally determined antigenic regions on the query sequence, (ii) creation of allele-specific peptide data set, and (iii) BLAST search against MHC or antigen databases.
date: 2007
date_type: published
publication: Methods in molecular biology (Clifton, N.J.)
volume: 409
publisher: Springer Science
pagerange: 95-112
refereed: TRUE
issn: 1064-3745
official_url: http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-60327-118-9_6
citation:   Bhasin, Manoj and Lata, Sneh and Raghava, G.P.S.  (2007) Searching and mapping of T-cell epitopes, MHC binders, and TAP binders.  Methods in molecular biology (Clifton, N.J.), 409.  pp. 95-112.  ISSN 1064-3745     
document_url: http://crdd.osdd.net/open/61/1/raghava2007.pdf